Journal: Nutrients

Article Title: Asiatic Acid Prevents the Deleterious Effects of Valproic Acid on Cognition and Hippocampal Cell Proliferation and Survival

doi: 10.3390/nu8050303

Figure Lengend Snippet: The number of Ki-67 positive cells in the SGZ of the hippocampal dentate gyrus. Ki-67 positive cells were stained in the SGZ of the dentate gyrus (green; ( A )). All nuclei were counterstained with propidium iodide (red; ( B ) and figure merged ( C )). The number of proliferating cells in animals receiving only VPA was significantly lower (* p < 0.05) than vehicle, Asiatic acid and Asiatic acid plus VPA groups, while Asiatic acid and Asiatic acid plus VPA groups were significantly higher when compared to the vehicle group (# p < 0.05). One-way ANOVA with LSD post hoc test was used to compare between all groups ( D ).

Article Snippet: Sections ware fixed in 0.5% paraformaldehyde (pH 7.4) for 3 min and then sections were incubated with primary antibody monoclonal mouse Ki-67 (1:150, Vector Laboratory, Inc., Burlingame, CA, USA) at room temperature for 1 h. Sections were then incubated with secondary antibody Alexa 488 rabbit anti mouse IgG (1:300, Invitrogen, Carlsbad, CA, USA) for 40 min and counter stained with propidium iodide (PI) (1:6000, Sigma Aldrich, Inc., St. Louis, MO, USA) for 30 s and mounted in glycerol.

Techniques: Staining

Journal:

Article Title: Puma is required for p53-induced depletion of adult stem cells

doi: 10.1038/ncb2100

Figure Lengend Snippet: Depletion of adult neural stem cells in the dentate gyrus of p53TSD/− mice, which is suppressed by Puma-deficiency. (a) Representative images of sections taken from the brains of 30-day-old p53+/+-, p53+/−-, p53TSD/−-and p53TSD/− Puma−/−-littermates that were immunochemically stained for Ki67, doublecortin and NeuN. (b) Sections from a were immunochemically stained for Sox2 and Glial fibrillary acidic protein (GFAP) to identify the neural stem cells and neurons. Scale bar, 50 μm.

Article Snippet: The primary antibodies used were mouse anti-neuronal-specific nuclear protein (NeuN) antibody (1:200; Chemicon), goat anti-DCX (1:250; Santa Cruz Biotechnology), rabbit anti-Ki67 (1:500; Vector Laboratories), chicken anti-GFAP (1:1000; Chemicon) and rabbit anti-Sox2 (1:200; Chemicon).

Techniques: Staining

Journal: PLoS ONE

Article Title: Glioblastoma Models Reveal the Connection between Adult Glial Progenitors and the Proneural Phenotype

doi: 10.1371/journal.pone.0020041

Figure Lengend Snippet: (A–C) Schematic views show the distribution of YFP+ cells (green dots) at 3 dpi (A), 7 dpi (B) and 21 dpi (C). (D–F) Triple immunofluorescence shows YFP (green) ki67 (red) and Olig2 (blue) at 3 dpi (D), 7 dpi (E) and 21 dpi (F). The arrows in D and E mark triple positive cells. The tables below each panel show the counts of ki67+/YFP+ cells at each time point (total, Olig2+, and Olig2−). (G–I) Triple immunofluorescence shows the expression of different markers in tumor cells at 21 dpi: (G) shows ki67 (red), YFP+ (green) and PDGFRα+ (blue). (H) shows Olig2 (red), YFP (green) and GFAP (blue). (I) shows Olig2 (red), YFP (green) and NeuN (blue). (J–O) Low power montage of 21 dpi tumor shows Olig2 (J), YFP (K), PDGFRα (L), Hoechst (M), GFAP (N) and Pten (O).

Article Snippet: Primary antibodies used were as follows: Mouse-GFAP (Chemicon), Rab-GFAP (Dacocytomation), Rab-Olig2 (Chemicon), Guinea pig-Olig2 (Gift of Dr. Tom Jessell), Rab-PDGFRα (Cell Signaling), Rab-Pten (Cell Signaling), Rab-GFP (Invitrogen), Chicken-GFP (Invitrogen), Rab-ki67 (Vector Lab), Rab-ki67 (Abcam), Guinea pig-NG2 (Gift of Dr. Bill Stallcup), Rab-NG2 (Chemicon), Goat-Doublecortin (Santa Cruz), Rab-Sox2 (Chemicon), Mouse-NeuN (Chemicon), Rab-Tcf4 (Millipore), Mouse-Nkx2.2 (DSHB), Rab-Sox10 (Sigma), Mouse-Nestin (Chemicon), Hoechst 33342 (Invitrogen).

Techniques: Immunofluorescence, Expressing